Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,181 results for: nes

Clear keywords
Showing: 7601 - 7620 of 16181 results

  1. lentiCRISPR - Amplicon, JAK2 sgRNA 1

    Plasmid
    #70679
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. WDR5 (2H9N)

    Plasmid
    #25229
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

    Enlarge

  6. Ydf NanoLuc

    Plasmid
    #225656
    Purpose
    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NanoLuciferase
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Promoter
    PydfJ115
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225656-plasmid-map-sequence-id-455061

    Enlarge

  13. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid
    #136405
    Purpose
    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.
    Depositor
    Massimo Squatrito
    Publication
    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    BbsI restriction site at position 437 was removed…
    Promoter
    U6, PDK
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136405-plasmid-map-sequence-id-269877

    Enlarge

  16. pMaCTag-Z03

    Plasmid
    #120046
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    moxCerulean3
    Use
    Pcr template
    Tags
    moxCerulean3
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120046-plasmid-map-sequence-id-232652

    Enlarge

  17. pMaCTag-P10

    Plasmid
    #120021
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKOk
    Use
    Pcr template
    Tags
    mKOk
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120021-plasmid-map-sequence-id-232619

    Enlarge

  18. pMaCTag-P01

    Plasmid
    #120012
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    moxBFP
    Use
    Pcr template
    Tags
    moxBFP
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120012-plasmid-map-sequence-id-232609

    Enlarge

Showing: 7601 - 7620 of 16181 results