We narrowed to 18,729 results for: Mut
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Plasmid#131717PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceNov. 11, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_WT_ADRB2_LucReporter
Plasmid#129788Purposeencodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G469A
Plasmid#131711PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-ND-Cdt1-mCherry
Plasmid#193761PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7 C450A
Plasmid#104628Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutationDepositorInsertCaspase-3 (CASP3 Human)
TagsHis and LOV2ExpressionBacterialMutationC450A (dark-locked and light-insensitive mutation)PromoterT7Available SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2*
Plasmid#220246PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2* (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPRKKKR494-499NAAIRSPromoterEF-1αAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594N
Plasmid#131713PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZYeGFP-SARS-CoV-2E GGG
Plasmid#224582PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cellsDepositorInsertSARS-CoV-2E GGG (E SARS-CoV-2)
TagsGFPExpressionMammalianMutationPBM (LLV) mutated to GGGPromoterCMVAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianPromoterCAGAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
Plasmid#22034DepositorInsertmVenus-PA-Rac1-C450M (RAC1 Avena sativa (oat), Human)
UseRetroviralExpressionMammalianMutationThe LOV domain contains the mutation C450M.Rac1 s…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
3322_pETcon_SARS2_Omicron-BA2
Plasmid#184409Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTEE
Plasmid#159748PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B
Plasmid#203695Purposeexpresses ATP10BDepositorInsertATP10B (ATP10B Human)
ExpressionMammalianAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
KCC2-pHext
Plasmid#104076PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagspHluorine tag inserted in the second putative tra…ExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PRKD1
Plasmid#116778PurposeLentiviral expression of PRKD1DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTUU
Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOX2 KO (T2A-H2B-EGFP)
Plasmid#167969PurposeSOX2 CDS replacement with T2A-H2B-EGFPDepositorInsertSRY-Box Transcription Factor 2 (SOX2 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only