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NET29A.Hs pEGFP-N2 (1181)

Plasmid

#62040

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET29A (TMEM120A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Splice variant

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-FKBP-LAP1

Plasmid

#34841

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FKBP-LAP1

Tags

His Tag, T7 tag, and myc

Promoter

CMV

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_short (EFS)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LiPOP1

Plasmid

#168264

Purpose

A single-component optogenetic tool for photoswitchable necroptosis

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

MLKL(1-125) (MLKL Human)

Tags

mCherry, CRY2-PHR

Expression

Mammalian

Mutation

H15A, K16A, R17A

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_blast-tagBFP-lox2272

Plasmid

#162071

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_blast-tagBFP-lox5171

Plasmid

#162072

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-Ty-HXGPRT-LoxP

Plasmid

#86667

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-Ty-HGPRT 3'UTR

Use

Expression in toxoplasma gondii

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDTnLAPP2A frame 0

Plasmid

#28226

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1260

Plasmid

#32282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1295

Plasmid

#32283

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-PKCG

Plasmid

#16180

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

PRKC gamma (PRKCG Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

Plasmid

#214994

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD55-T2A-GFP

Plasmid

#205459

Purpose

lentiviral plasmid for expression of human CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD55 (CD55 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-eGFP

Plasmid

#191104

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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