We narrowed to 8,554 results for: sgRNA

1. Cbh_v5 AAV-CBE N-terminal

   Plasmid

   #137175

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE N-terminal; U6-protospacer
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv4

   Plasmid

   #221432

   Purpose

   Lentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-sgAMPKa1-Cas9-GFP

   Plasmid

   #208049

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

   Depositor

   Naama Kanarek

   Article

   Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

   Insert

   sgPRKAA1 (PRKAA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-eSpCas9_sgAAVS1

   Plasmid

   #199213

   Purpose

   All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   AAVS1-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABE8e-NG-403Q-Cterm-AAV

   Plasmid

   #194652

   Purpose

   C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG C-term
   

   Use

   AAV

   Tags

   BPNLS and NpuC split intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pED9x (dCas9-KRAB-mCherry)

    Plasmid

    #163956

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

    Plasmid

    #199262

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HA.V5.VSVg_NGFR

    Plasmid

    #158244

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCKO

    Plasmid

    #73311

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid

    #99690

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5d.EFS.SpCas9.P2A.BSD

    Plasmid

    #57821

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPR v2-sgACSL4 clone2

    Plasmid

    #162129

    Purpose

    Lentiviral sgRNA plasmid targeting human ACSL4

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgACSL4 (ACSL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPR v2-sgACSL4 clone1

    Plasmid

    #162128

    Purpose

    Lentiviral sgRNA plasmid targeting human ACSL4

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgACSL4 (ACSL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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