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Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, EF-1a

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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PME-1 pcDNA-StrepIII

Plasmid

#119292

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Expression

Mammalian

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5b.z-AP-His

Plasmid

#72053

Purpose

Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b.z (Unc5b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

Plasmid

#188391

Purpose

Reporter for BCAM expression

Depositor

Markus Frank

Article

Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

Insert

BCAM reporter

Use

Luciferase

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Talin1 head EGFP fusion

Plasmid

#221408

Purpose

Mammalian expression of Talin1 head EGFP fusion for mammalian expression

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

Talin1 head EGFP fusion (TLN1 Human)

Expression

Mammalian

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-EGFP-SOS1

Plasmid

#178860

Purpose

Mammalian expression of SOS1 fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

SOS1 (SOS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

Plasmid

#229788

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mGreenLantern; ZF10-NS3-p65

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF10 and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB)

Plasmid

#169721

Purpose

Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSF-1Rwt-IRES-GFP

Plasmid

#115523

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

human CSF-1 receptor (CSF1R Human)

Use

Retroviral

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG PIK3CA E545K

Plasmid

#73055

Purpose

Human PIK3CA E545K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA (PIK3CA Human)

Expression

Mammalian

Mutation

E545K

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (WT)

Plasmid

#197008

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

-

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp140-TM

Plasmid

#123251

Purpose

Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Tags

CD5 leader peptide and Linker (with 6his tag) and…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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