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pLV Ptdss2-V5

Plasmid

#175143

Purpose

Lentiviral expression of mouse Ptdss2-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Ptdss2 (Ptdss2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux_crRNA NR1

Plasmid

#176244

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a_crRNA NC

Plasmid

#176256

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A IRES GFP

Plasmid

#133818

Purpose

Encodes the C2A domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 E 27nt-del_nostop

Plasmid

#153955

Purpose

Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTetBl-CLIP-CP110

Plasmid

#136851

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-CP110

Plasmid

#136803

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Cep70

Plasmid

#136871

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep70 (CEP70 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep70

Plasmid

#136870

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep70 (CEP70 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-STIL

Plasmid

#136844

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-STIL (STIL Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep41

Plasmid

#136843

Purpose

Mammalian expression of the centrosomal protein Cep41 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep41 (CEP41 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep164

Plasmid

#136824

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep164 (CEP164 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-KIAA0841

Plasmid

#136810

Purpose

Mammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-KIAA0841 (HAUS5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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ETV3_pet28a

Plasmid

#131646

Purpose

Bacterial expression of ETS gene ETV3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3 (ETV3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Promoter

Ef1a

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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