We narrowed to 26,306 results for: Vit

1. pAAV-shCTRL

   Plasmid

   #181875

   Purpose

   Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   control shRNA with no known targets in the mouse genome
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EYFP-hDF-deltaTM-pCMV-9

   Plasmid

   #72586

   Purpose

   human dysferlin no transmembrane domain tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin (DYSF Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   transmembrane domain deleted

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ Zf GFP-vclb

   Plasmid

   #185411

   Purpose

   GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin b (vclb Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. VRQR variant

   Plasmid

   #138562

   Purpose

   Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p207-Switch-ON (FRT)

   Plasmid

   #217886

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-mCherry-mTrim21ΔPRYSPRY

   Plasmid

   #105524

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB6-CDC34-HA (pRKB261)

   Plasmid

   #99145

   Purpose

   Mammalian expression construct to express HA-tagged CDC34

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   CDC34 (CDC34 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pTight

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. OPRD1-DuET

    Plasmid

    #213358

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRD1 (OPRD1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1-USP11

    Plasmid

    #78849

    Purpose

    To purify GST-tagged recombinant USP11 protein

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

    Insert

    USP11 (USP11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pPBC-G6s

    Plasmid

    #62812

    Purpose

    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6s-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG/ATP11A(E186Q)-HA

    Plasmid

    #209249

    Purpose

    Mammalian expression of ATP11A mutant E186Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11A (ATP11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E186Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT121_GalL_nCDA1-VQRBE3

    Plasmid

    #145086

    Purpose

    Expressing base editor nCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VQRBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P2RY11-DuET

    Plasmid

    #213364

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    P2RY11 (P2RY11 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. STARR-seq luciferase validation vector_mP_empty

    Plasmid

    #99298

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-basic-hRARβP-FL-luciferase

    Plasmid

    #135447

    Purpose

    Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Full-length promoter of human retinoic acid receptor-beta gene
    

    Promoter

    Human RARβ promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEYFP-hDF C2B

    Plasmid

    #72583

    Purpose

    human dysferlin C2B domain tagged with YFP

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin C2B domain (DYSF Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C2B domain

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pScalps_puro_mIL-2Rα

    Plasmid

    #59917

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only