We narrowed to 9,972 results for: crispr plasmids

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pDonor NTRK1-P2A-tdTomato

Plasmid

#246653

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human NTRK1 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by NTRK1 homology regions (NTRK1 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Mutation

tdTomato was altered to contain a silent SAL1 res…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDS48

Plasmid

#241839

Purpose

Cas9-gRNA construct targeting the UBE3A locus

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

Cas9-gRNA (UBE3A )

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSCBE3-HF1

Plasmid

#218156

Purpose

This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-HF1
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N497A…

Promoter

rpsL promoter

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCBE3-Hypa

Plasmid

#218157

Purpose

This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N692A…

Promoter

rpsL promoter

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1003K

Plasmid

#207371

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1003K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A848K

Plasmid

#207369

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A848K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A661R

Plasmid

#207367

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-Q661R

Plasmid

#207366

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-Q661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, K526E, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-N515Y

Plasmid

#207364

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-N515Y

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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HypaR-SpCas9

Plasmid

#126757

Purpose

Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

Plasmid

#199451

Purpose

CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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