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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBASH3B-1/1

Plasmid

#91462

Purpose

Protein expression and purification of human SH3 domain construct UBASH3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3B-1/1 (UBASH3B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SHANK3-1/1

Plasmid

#91421

Purpose

Protein expression and purification of human SH3 domain construct SHANK3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK3-1/1 (SHANK3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;basPEgfp

Plasmid

#90169

Purpose

contains a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

basalPEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_WT

Plasmid

#81903

Purpose

Gateway Donor vector containing PIK3R3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB4_WT

Plasmid

#81766

Purpose

Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SERPINB4 (SERPINB4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_p.R161P

Plasmid

#81671

Purpose

Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway Cloning

Mutation

R161P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_p.H115Y

Plasmid

#81658

Purpose

Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway Cloning

Mutation

H115Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTPN12_p.E57D

Plasmid

#81528

Purpose

Gateway Donor vector containing PTPN12 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Mutation

E57D; V322I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.R5Q

Plasmid

#81294

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

R5Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKAA1_WT

Plasmid

#82274

Purpose

Gateway Donor vector containing PRKAA1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway Cloning

Mutation

M10L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VEGFC_WT

Plasmid

#82282

Purpose

Gateway Donor vector containing VEGFC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VEGFC (VEGFC Human)

Use

Gateway Cloning

Mutation

K414Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K3_WT

Plasmid

#82140

Purpose

Gateway Donor vector containing MAP2K3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K3 (MAP2K3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_47

Plasmid

#60071

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
RhoH

Plasmid

#55697

Purpose

expression of mouse RhoH

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoH (Rhoh Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RRAS2

Plasmid

#55660

Purpose

expression of mouse RRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS2 (Rras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
GRASP

Plasmid

#38808

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GRASP (TAMALIN Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
FmnI GST369/pGEX6p1

Plasmid

#19322

Depositor

Philip Leder

Article

Zhou et al Exp Cell Res. 2006 Apr 15. 312(7):1119-2

Insert

ForminI Exon 9 (Fmn1 Mouse)

Expression

Bacterial

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only