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R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Plasmid

#159563

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLN397 (FuGW-S(E2F1)p-Sponge7)

Plasmid

#105181

Purpose

Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLN431 (FuGW-G8p-mKate2-1Pe )

Plasmid

#105186

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

Plasmid

#105192

Purpose

Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pH9-ilvA-ccdBwt (DE25)

Plasmid

#135170

Purpose

Expressing synthetic overlapping gene, ilvA-ccdB

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

ilvA-ccdB

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-5

Plasmid

#135169

Purpose

Expressing cysJ-infA functional variant 5

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-5

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-6

Plasmid

#135168

Purpose

Expressing cysJ-infA functional variant 6

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-6

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-4

Plasmid

#135167

Purpose

Expressing cysJ-infA functional variant 4

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-4

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-3

Plasmid

#135166

Purpose

Expressing cysJ-infA functional variant 3

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-3

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-2

Plasmid

#135165

Purpose

Expressing cysJ-infA functional variant 2

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-2

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CI-1

Plasmid

#135164

Purpose

Expressing cysJ-infA functional variant 1

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

CysJ-infA overlapping region in CI-1

Use

Synthetic Biology

Promoter

TetO

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pH9-ilvA-acp (DE2)

Plasmid

#135163

Purpose

Expressing synthetic overlapping gene, ilvA-acpP

Depositor

Harris Wang

Article

Blazejewski et al Science. 2019 Aug 9;365(6453):595-598. d

Insert

ilvA-acp

Use

Synthetic Biology

Promoter

TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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L4276 hproTGFb1 in pcDNA backbone

Plasmid

#244183

Purpose

Constitutive expression of human pro-TGF-beta1 (with its signal sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

pro-TGF-beta1 (TGFB1 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

Plasmid

#139230

Purpose

mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPD128 AND Gate, 3 repeats

Plasmid

#138937

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 3 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 3 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD392 VPR-ZF92

Plasmid

#138841

Purpose

Constitutive expression of VPR-ZF92 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF92

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPD303 VP64-ZF43

Plasmid

#138835

Purpose

Constitutive expression of VP64-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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