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pDMJ2-SARS-CoV-2-Spike (B.1-9b)

Plasmid

#241052

Purpose

Expresses SARS-CoV-2 spike (B.1-9b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,T478K,E484V,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8c)

Plasmid

#241049

Purpose

Expresses SARS-CoV-2 spike (B.1-8c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8d)

Plasmid

#241050

Purpose

Expresses SARS-CoV-2 spike (B.1-8d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8a)

Plasmid

#241047

Purpose

Expresses SARS-CoV-2 spike (B.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,R158A,K147N,N439R,T478K,S494R,F490R,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-8b)

Plasmid

#241048

Purpose

Expresses SARS-CoV-2 spike (B.1-8b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R403T,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-5a)

Plasmid

#241046

Purpose

Expresses SARS-CoV-2 spike (B.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,N481T,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-hs_UbiC-pro (JDW 1461)

Plasmid

#242547

Purpose

Enhancer/Promoter: Gateway 5' entry vector containing human Ubc promoter (-1225 to -6)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Ubiquitin / Ubc promoter (UBC Human)

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-Myc (JDW 1441)

Plasmid

#242569

Purpose

Gateway middle entry clone containing EGFP fused to oncogenic c-Myc.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-Myc (MYC Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-SUMO-m-cGAS(I309T)

Plasmid

#232295

Purpose

Expression of mutant mouse cGAS protein (I309T)

Depositor

Thomas Tuschl

Article

Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

Insert

MB21D1 (Cgas Mouse)

Expression

Bacterial

Mutation

I309T

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-T2A-Isl1

Plasmid

#233161

Purpose

Retroviral expression of mNgn2x3HA and Isl1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-T2A-mLhx3

Plasmid

#233162

Purpose

Retroviral expression of mNgn2x3HA and Lhx3

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-T2A-Lhx3 (Neurog2, Isl1 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Crestin-pro (JDW 1320)

Plasmid

#229830

Purpose

A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Crestin promoter, c-fos minimal promoter, and b-globin intron

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K4-10A

Plasmid

#229403

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K4 (MAP4K4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S523,S536,S547,S643,T684,S726…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K4-10D

Plasmid

#229404

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K4 (MAP4K4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S523,S536,S547,S643,T684,S726…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgPet-1-hSyn-mCherry-KASH

Plasmid

#223227

Purpose

guideRNA targeting the mouse Pet-1 (Fev)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Fev (Fev Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut1-Luc

Plasmid

#223664

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 1

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

mTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut2-Luc

Plasmid

#223665

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 2

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

mTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut3-Luc

Plasmid

#223666

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 3

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

mTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut4-Luc

Plasmid

#223667

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 4

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

mTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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