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We narrowed to 24,135 results for: CRISPR

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Showing: 11611 - 11640 of 24135 results

  1. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  3. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  6. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  9. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  12. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  13. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  14. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

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  15. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Pim Pijnappel
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159352-plasmid-map-sequence-id-314976

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  16. Protein2PAM_NmeQ981A

    Plasmid
    #250230
    Purpose
    Express Protein2PAM_NmeQ981A nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeQ981A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Q981A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250230-plasmid-map-sequence-id-501768

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  17. Protein2PAM_NmeN1029A

    Plasmid
    #250229
    Purpose
    Express Protein2PAM_NmeN1029A nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN1029A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 N1029A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250229-plasmid-map-sequence-id-501766

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  18. AgU6.695_3xP3-DsRed1-SV40 (Single gRNA)

    Plasmid
    #243009
    Purpose
    For cloning a single gRNA after BspQI digestion under An. gambiae U6.695 promoter.
    Depositor
    George Christophides
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Insect
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    243009-plasmid-map-sequence-id-486379

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  19. pKS1603

    Plasmid
    #239527
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239527-plasmid-map-sequence-id-479035

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  20. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

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  21. pKS1615

    Plasmid
    #239546
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239546-plasmid-map-sequence-id-478363

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  22. pKS1613

    Plasmid
    #239531
    Purpose
    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239531-plasmid-map-sequence-id-475886

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  23. 1203R-CMV-3'dmDR-EGFP-bGHpolyA

    Plasmid
    #220962
    Purpose
    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220962-plasmid-map-sequence-id-437429

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  24. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

    Plasmid
    #221454
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221454-plasmid-map-sequence-id-437443

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  25. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

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  26. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    BP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165416-plasmid-map-sequence-id-334022

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  27. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid
    #191391
    Purpose
    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hCdt1(30-120)
    Use
    CRISPR and Synthetic Biology
    Tags
    mScarlet-I
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191391-plasmid-map-sequence-id-378101

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  28. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  29. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  30. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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Showing: 11611 - 11640 of 24135 results