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pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

Plasmid

#164018

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C

Plasmid

#164023

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E490R

Plasmid

#164027

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E490R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R495E

Plasmid

#164028

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R495E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C D511K

Plasmid

#164029

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, D511K

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C Y527A

Plasmid

#164030

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HBT-CPK10ac-Flag

Plasmid

#107427

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK10 (CDPK1 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK10-Flag

Plasmid

#107428

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK10 (CDPK1 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK30ac-Flag

Plasmid

#107429

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK30 (CPK30 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK30-Flag

Plasmid

#107430

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK30 (CPK30 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32ac-Flag

Plasmid

#107431

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Snrnp40

Plasmid

#134255

Purpose

Lentivector for Snrnp40 CRISPR knockout

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

CRISPR and Lentiviral

Mutation

Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

Promoter

U6

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-Snrnp40

Plasmid

#134249

Purpose

Lentivector encoding Flag-tagged Snrnp40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-A

Plasmid

#138672

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK2-A

Plasmid

#138670

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-C

Plasmid

#138674

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECC-sgSIK1-D

Plasmid

#138668

Purpose

Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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