We narrowed to 866 results for: seph

1. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

   Plasmid

   #133790

   Purpose

   CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized NmeCas9
   

   Use

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV10/3xflag-Clock L57E/L113E/W284A

   Plasmid

   #47343

   Purpose

   triple mutation used for co-IP

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   L57E/L113E/W284A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3-HA/Bmal I317D

   Plasmid

   #47347

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   I317D

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3-HA/Bmal L115E

   Plasmid

   #47345

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L115E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

5. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

   Plasmid

   #65806

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Use

   Tags

   4 MS2 stem loops, SMG5 3'UTR shortened versi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-HA/Bmal L95E/L150E/W427A

   Plasmid

   #47352

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E/L150E/W427A

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3-HA/Bmal W427A

   Plasmid

   #47349

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   W427A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. HLH Clock Pas 1-401 VenN

   Plasmid

   #47353

   Purpose

   Clock fragment cloned and tagged with VenN

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3-HA/Bmal V435R

   Plasmid

   #47350

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   V435R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. HLH Clock Pas 1-401 VenN L74E

    Plasmid

    #47355

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L74E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3-HA/Bmal L150E

    Plasmid

    #47346

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L150E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. HLH Bmal Pas 1-465-VenC

    Plasmid

    #47364

    Purpose

    Bmal fragment cloned with Venus tag and used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGL4.0-TERT_-100_-79

    Plasmid

    #193785

    Purpose

    luciferase reporter for duplicated TERT promoter

    Depositor

    Joseph Costello

    Article

    Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

    Plasmid

    #133796

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1 (truncated C-terminal domain only)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    C-terminal domain only

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

    Plasmid

    #133798

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T114A/F115A)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T114A/F115A

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid

    #133791

    Purpose

    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St1Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V1 sgRNA architecture from Carter et al. biorxiv …

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid

    #133792

    Purpose

    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St3Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Muller et al. Molecular T…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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