2,226 results

1. pFSW Syt1 WT IRES GFP

   Plasmid

   #133816

   Purpose

   Encodes full-length wild type synaptotagmin 1 for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHLSec2-irisin-his

   Plasmid

   #122729

   Purpose

   Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Bovine, Mouse)
   

   Use

   Tags

   his and signal sequence for secretion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-beta-arrestin1

   Plasmid

   #36918

   Depositor

   Robert Lefkowitz

   Article

   Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

   Insert

   beta-arrestin1 (Arrb1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Kv4.3 long/RBG4

   Plasmid

   #196946

   Purpose

   Plasmid expressing an antigen targeting the Kv4.3 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv4.3 (Kcnd3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-syn-fRCaMP2

   Plasmid

   #125246

   Purpose

   Expresses red fluorescent calcium sensor in hippocampal slices

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRCaMP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D396A

   Promoter

   synapsin

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KvB2/RBG4

   Plasmid

   #196942

   Purpose

   Plasmid expressing an antigen targeting the Kvbeta2 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   KvB2/B6 (Kcnab2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK280 (mCherry2-NeoR)

   Plasmid

   #72796

   Purpose

   C-terminal tagging with mCherry2 using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mCherry2-Bsr
   

   Use

   CRISPR

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE Rims1-GFP KI

   Plasmid

   #131494

   Purpose

   Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rims1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP SV2A GFP

   Plasmid

   #218544

   Purpose

   Mamallian Expression of SV2A GFP

   Depositor

   Edwin Chapman

   Article

   Dong et al Science. 2006 Apr 28;312(5773):592-6. do

   Insert

   SV2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S42F

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSynapsin_NLS_GFP_P2A_SNAP_NR1

    Plasmid

    #170458

    Purpose

    Expresses SNAP tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAL4-CREB

    Plasmid

    #15221

    Depositor

    Ugo Moens

    Article

    Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pORANGE GFP-RAB11a KI

    Plasmid

    #131499

    Purpose

    Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rab11a, Rab11a Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACT2-μ3B

    Plasmid

    #198175

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAMPK alpha2 delta312X

    Plasmid

    #60127

    Purpose

    expresses constitutively active AMPKalpha2

    Depositor

    Morris Birnbaum

    Insert

    AMPK alpha 2 (Prkaa2 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Truncated at residue 312

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Syn-H2B-jGCaMP8m-WPRE

    Plasmid

    #176748

    Purpose

    AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    H2B-jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX4T3-Stx3(1-265)

    Plasmid

    #100573

    Purpose

    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.

    Depositor

    Thomas Weimbs

    Insert

    Stx3-1-265 (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain, residues 4-264 present.

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Halo-p58

    Plasmid

    #166896

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L405Q, R419S- please see depositor comments

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-PRKAA2

    Plasmid

    #30310

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α2 (Prkaa1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. G protein alpha-s(alpha3beta5)

    Plasmid

    #55799

    Purpose

    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…

    Promoter

    CMV

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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