We narrowed to 5,034 results for: plasmid dna
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Plasmid#19782DepositorTypeEmpty backboneUseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only
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AAV-SYN-integrin alpha9-V5
Plasmid#203729PurposeAAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoterDepositorInsertITGA9 (ITGA9 Human)
UseAAVTagsV5-tagExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- κβ_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-κβ DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorInsertGLI1 (Gli1 Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS2
Plasmid#46954PurposeCloning plasmid for the generation of Knock-In in human cells using rAAV.DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc
Plasmid#16539Purposeluciferase reporter plasmid with very low basal activityDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverespression of Flp step-arrest mutant in yeast, URA selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(Trp1)
Plasmid#49459Purposeoverespression of Flp step-arrest mutant in yeast, TRP1 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP
Plasmid#10676PurposeRetroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAP1638
Plasmid#137089PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)DepositorInsert4*(GFP11)
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP1836
Plasmid#137087PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP1837
Plasmid#137086PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP3041
Plasmid#137083PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFPDepositorInsertGFP11::3xFlag::tagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP1680
Plasmid#137088PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFPDepositorInserttagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-GFPd2
Plasmid#115665PurposePiggybac transposon plasmid for destabilized eGFP (GFPd2)DepositorInsertGFPd2
UseSynthetic BiologyTagsDestabilizationExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10-WPRE-UbC-Emerald
Plasmid#203804PurposeLentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-Llgl1-WPRE-UbC-Emerald
Plasmid#203805PurposeLentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pexA2_ring
Plasmid#89672PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.DepositorInsertring
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_cross
Plasmid#89677PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the cross shown in figure 3G in the associated article.DepositorInsertcross
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_drigalski
Plasmid#89676PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the Drigalski spatula shown in figure 3E of associated article.DepositorInsertdrigalski
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_square
Plasmid#89674PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the square shown in figure 3F of associated article.DepositorInsertsquare
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_wm_ruleB
Plasmid#89675PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the three-armed star shown in figure 3D of associated article.DepositorInsertwm_ruleB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-Mut6E
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVDnmt3A_bGHpA
Plasmid#177349PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVDnmt3A_bGHpA
Plasmid#177355PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtnluc
Plasmid#184339PurposeTransformation of mitochondrial DNA in yeastDepositorInsertnanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pexA2_2hb_ruleB
Plasmid#89670PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the two-helix-bundle shown in figure 2E,F,G of associated article.DepositorInsert2hb_ruleB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_4hb_straight
Plasmid#89678PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the straight four-helix-bundle shown in figure 4A of associated article.DepositorInsert4hb_straight
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_4hb_bent
Plasmid#89679PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent four-helix-bundle shown in figure 4E of associated article.DepositorInsert4hb_bent
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexK4_bow_360
Plasmid#89673PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3B of associated article.DepositorInsertbow_360
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pexA2_bow_180
Plasmid#89671PurposePlasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3A of associated article.DepositorInsertbow_180
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3CTc3A(1-135)
Plasmid#63138PurposeExpression of human Tc3(1–135) bipartite domain tagged with 6xHisDepositorInsertTc3A
UseTags6xHisExpressionBacterialMutation1-135 aa bipartite domainPromoterT7Available sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 CBS4 (GB2718)
Plasmid#160601PurposeCopper dependent CUP2 binding DNA sequenceDepositorInsertCBS4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX_403
Plasmid#41395PurposeInducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTADepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsV5ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only