Addgene

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pT2Aneo-mRFP-FKBP-mSos1-linkercat

Plasmid

#173858

Purpose

Encoding a part of the rapamycin-induced Ras activation system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

mRFP-FKBP-mSos1-linkercat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dNrg1

Plasmid

#173696

Purpose

A knockout vector for the dog Nrg1.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEreg

Plasmid

#173697

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dTgfa

Plasmid

#173698

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dHbegf

Plasmid

#173699

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-dEgf

Plasmid

#173840

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisHbegf

Plasmid

#173846

Purpose

Encoding dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Hbegf.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1HR1AtR2NtB2

Plasmid

#160890

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-S1H

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

rbcS-SH1: T7, chaperones: T13

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT4aR1AtR2NtB2

Plasmid

#160887

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T4a

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-T4a: T7, chaperones: T10

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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gamma-tubulin (334-451)

Plasmid

#104436

Purpose

expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin fragment 334 to 451 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

deleted a.a. 1 to 333

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00305

Plasmid

#34431

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10021 (bowl Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Expression

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

Plasmid

#67294

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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