Addgene

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pBIG1a_GST-TEV-CK2alpha'_CK2beta

Plasmid

#223741

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

CK2-alphaprime_CK2-beta (CSNK2A2 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC (Y530F)

Plasmid

#223743

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

Src Kinase (SRC Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Y530F constitutively active mutant

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST

Plasmid

#223744

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BCL2L13 soluble part (BCL2L13 Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3A

Plasmid

#223726

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

LC3A (MAP1LC3A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-120 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL1

Plasmid

#223729

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL2

Plasmid

#223730

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT)

Plasmid

#223733

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-182 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT)

Plasmid

#223734

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FUNDC1 soluble part (FUNDC1 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-50 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPA1_D262V-FLAG-IRES-eGFP

Plasmid

#234620

Purpose

Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 D262V (HNRNPA1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Famillial ALS mutation D262V, C-terminal FLAG tag…

Promoter

CMV

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

Plasmid

#212423

Purpose

Encodes SARS-CoV-2 spike E484Q for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

E484Q

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

Plasmid

#212486

Purpose

Encodes SARS-CoV-2 spike L981F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

L981F

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T572I (ATC)

Plasmid

#212404

Purpose

Encodes SARS-CoV-2 spike T572I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T572I (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

T572I

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

Plasmid

#229795

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T29I (ATA)

Plasmid

#212403

Purpose

Encodes SARS-CoV-2 spike T29I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T29I (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

T29I

Promoter

CAG

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q183H (CAT)

Plasmid

#223279

Purpose

Encodes SARS-CoV-2 spike Q183H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q183H (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q183H

Promoter

CAG

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R204A

Plasmid

#222196

Purpose

Bacterial expression of mouse PTER-R204A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R204A

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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