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pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP

Plasmid

#99730

Purpose

overexpression of Tmeff2 and mCherry

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

Tags

2xMyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP

Plasmid

#99729

Purpose

overexpression of Tmeff2 and CopGFP

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p415TEF-NQYFP

Plasmid

#99554

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTW087

Plasmid

#64756

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_63/pRSETB

Plasmid

#51980

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-KG/Net1A

Plasmid

#28261

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
PCDNA3 T7 DLC2

Plasmid

#24268

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
myc-Orai1 E190A pHAGE

Plasmid

#22753

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E190A (ORAI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E190A

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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human STIM1 CFP

Plasmid

#19755

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pJOG1368

Plasmid

#167301

Purpose

Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

FCY-UPP fusion gene

Use

Synthetic Biology; Moclo level 0 vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJOG1336

Plasmid

#167303

Purpose

Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

3'UTR and Terminator SlOLE2

Use

Synthetic Biology; Moclo level 0 vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJOG1334

Plasmid

#167302

Purpose

Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

Promoter SlOLE2

Use

Synthetic Biology; Moclo level 0 vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-cilia-APEX

Plasmid

#73186

Purpose

Expresses cilia-APEX in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

cilia-APEX (Nphp3 Mouse, Glycine max)

Use

Tags

internal GFP tag

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Mfn1-Myc

Plasmid

#23212

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Use

Tags

6xHis, 9xMyc, and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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ANO6-Plvx-mCherry-c1

Plasmid

#62554

Purpose

Full length mouse ANO6 tagged with mCherry on the C-terminus

Depositor

Renzhi Han

Article

Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

Insert

mouse ANO6 (Ano6 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-TRAF6-wt

Plasmid

#21624

Depositor

John Kyriakis

Article

Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

Insert

TNF receptor-associated factor 6 (Traf6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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