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We narrowed to 984 results for: appa

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Showing: 761 - 780 of 984 results

  1. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  2. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  9. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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  15. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid
    #238169
    Purpose
    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    CD45 sgRNA CRISPRa (PTPRC Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238169-plasmid-map-sequence-id-474451

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  16. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201955-plasmid-map-sequence-id-405309

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Showing: 761 - 780 of 984 results