We narrowed to 11,964 results for: cel.2;

1. pCAG-OSF-ATG13 (2-197)-E83L

   Plasmid

   #188099

   Purpose

   Expresses human ATG13 HORMA E83L mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-E83L

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-OSF-ATG13 (2-197)-Y115D

   Plasmid

   #188100

   Purpose

   Expresses human ATG13 HORMA Y115D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-Y115D

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-OSF-ATG13 (2-197)-F19D

   Plasmid

   #188097

   Purpose

   Expresses human ATG13 HORMA F19D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-F19D

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-ATG13 (2-197)-L151D

   Plasmid

   #188102

   Purpose

   Expresses human ATG13 HORMA L151D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-L151D

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS SARS-CoV-2 D614G Spike

   Plasmid

   #185692

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 D614G Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 D614G Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutation: D614G

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAGGS SARS-CoV-2 Delta Spike

   Plasmid

   #185593

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Delta Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Delta Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19R, Δ156-157,…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCAGGS SARS-CoV-2 Alpha Spike

   Plasmid

   #185691

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Alpha Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: Δ69-70, Δ145, N…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 USF2

    Plasmid

    #67069

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    USF2 (USF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 SREBF2

    Plasmid

    #67129

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SREBF2 (SREBF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 TFDP2

    Plasmid

    #67088

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFDP2 (TFDP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBS-KS-attB2-SA(2)-T2A-Gal4

    Plasmid

    #62901

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1 puro shTEAD1/3/4-2

    Plasmid

    #193673

    Purpose

    Constitutive lentiviral expression of TEAD shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    TEAD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid

    #24417

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Rs1
    

    Use

    Entry vector

    Tags

    FLAG and mCherry

    Expression

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-TRS-Leader-SARS-CoV-2-EGFP

    Plasmid

    #171585

    Purpose

    Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    TRS-Leader-SARS-CoV-2-d2eGFP
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

    Plasmid

    #49257

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Use

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Use

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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