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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,526 results for: construct

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Showing: 761 - 780 of 8526 results

  1. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  2. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

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  3. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

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  4. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  5. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  6. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  7. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  9. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  10. pARID4B.1.0-gDNA

    Plasmid
    #132436
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ARID4B (ARID4B Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132436-plasmid-map-sequence-id-266218

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  11. pCSRNP3.1.0-gDNA

    Plasmid
    #132472
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CSRNP3 (CSRNP3 Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132472-plasmid-map-sequence-id-267179

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  12. pHOXB6.1.0-gDNA

    Plasmid
    #132458
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    HOXB6 (HOXB6 Human)
    Use
    CRISPR
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132458-plasmid-map-sequence-id-266992

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  13. pGTF2I.1.0-gDNA

    Plasmid
    #132452
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    GTF2I (GTF2I Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132452-plasmid-map-sequence-id-266821

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  14. pHMG20B.1.0-gDNA

    Plasmid
    #132449
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    HMG20B (HMG20B Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132449-plasmid-map-sequence-id-266806

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  15. pMECOM.1.0-gDNA

    Plasmid
    #132445
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    MECOM (MECOM Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132445-plasmid-map-sequence-id-266786

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  16. pKLF10.1.0-gDNA

    Plasmid
    #132433
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    KLF10 (KLF10 Human)
    Use
    CRISPR
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132433-plasmid-map-sequence-id-266215

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  17. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

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  18. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  19. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

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  20. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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Showing: 761 - 780 of 8526 results