We narrowed to 4,657 results for: cre encoding plasmid

1. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

   Plasmid

   #177669

   Purpose

   Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

   Depositor

   Karl Deisseroth

   Article

   Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lox2272 Neo* lox2272

   Plasmid

   #168769

   Purpose

   lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBCSK_LEU2_I

   Plasmid

   #109060

   Purpose

   yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes

   Depositor

   Colin Logie

   Insert

   LEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   LEU2

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. loxP-T2A-Myr-Casp8-ERT2-rox

   Plasmid

   #182362

   Purpose

   Shuttle vector for T2A-Myr-Casp8-ERT2

   Depositor

   Mario Capecchi

   Insert

   loxP-T2A-Myr-Casp8-ERT2-rox
   

   Use

   Cre/Lox and Mouse Targeting; Dre / rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. loxP-T2A-Myr-DeadCasp8-ERT2-rox

   Plasmid

   #182361

   Purpose

   Shuttle vector for T2A-Myr-DeadCasp8-ERT2

   Depositor

   Mario Capecchi

   Insert

   loxP-T2A-Myr-DeadCasp8-ERT2-rox
   

   Use

   Cre/Lox and Mouse Targeting; Dre / rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

   Plasmid

   #172477

   Purpose

   sfGFP with a synthetic intron derived from the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

   Plasmid

   #172478

   Purpose

   sfGFP with a synthetic intron from the pCI expression vector (Promega)

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

   Plasmid

   #172475

   Purpose

   sfGFP with a synthetic intron derived from the mouse Ighe gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

   Plasmid

   #172476

   Purpose

   sfGFP with a synthetic intron derived from the rabbit beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid

    #178429

    Purpose

    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    Edward Callaway

    Inserts

    TVA950 Glu66→Thr
    Optimized G
    EGFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA0250-mCherry-2a-hM4D-nrxn1a rev

    Plasmid

    #60544

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Inserts

    mCherry
    muscarinic receptor 4, axon targeted variant (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid

    #196677

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIα-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid

    #196678

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIαmut-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-VTKS5-TVA-eGFP-N2cG

    Plasmid

    #175440

    Purpose

    Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSECC

    Plasmid

    #60820

    Purpose

    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

    Depositor

    Tyler Jacks

    Article

    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

    Inserts

    Cas9
    Cre
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BsmBI site eliminated by C->A; E308G

    Promoter

    EFS and EFS (after Cas9-2A)

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    TVA mammalian codon optimized

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid

    #200660

    Purpose

    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLP-mOlig2-ires-gfp

    Plasmid

    #49399

    Purpose

    mammalian bicistronic expression of mouse Olig2 and EGFP

    Depositor

    Randall McKinnon

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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