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pKT-iRFP-KAN

Plasmid

#64687

Purpose

pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

Depositor

Erin O'Shea

Publication

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

iRFP

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUC-UAS1B16-TEF(504)

Plasmid

#44371

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(504)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCMV-DAAO

Plasmid

#60344

Purpose

Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

Depositor

Vsevolod Belousov

Publication

Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

Insert

DAAO

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUpstream2Lox

Plasmid

#164573

Purpose

Integration of 2 LoxN sites in the genome of P. berghei

Depositor

Olivier Silvie

Inserts

Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Publication

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZS160 CRISPEY HDV-HDV NotI

Plasmid

#114453

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozyme

Depositor

Hunter Fraser

Publication

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Expression

Yeast

Promoter

ScGAL7p

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBEVY-GT

Plasmid

#51231

Purpose

bi-directional expression vector for yeast, galactose inducible, TRP1 selection

Depositor

Charles Miller

Publication

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBEVY-T

Plasmid

#51232

Purpose

bi-directional expression vector for yeast, constitutively active, TRP1 selection

Depositor

Charles Miller

Publication

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Expression

Yeast

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Publication

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PE

Plasmid

#92126

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Publication

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Promoter

Ppdc

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCP-tRNA

Plasmid

#133812

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

Depositor

Geraldine Butler

Publication

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter

Use

CRISPR

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BB3cH_pGAP_23*_pLAT1_Cas9

Plasmid

#104907

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BB3cK_pGAP_23*_pTEF_Cas9

Plasmid

#104909

Purpose

hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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