166,289 results

1. pET21a-TyrRS-His

   Plasmid

   #124121

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Tyrosyl-tRNA ligase (tyrS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Camui-CR

   Plasmid

   #40256

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Camuiα-CR
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-ValRS-His

   Plasmid

   #124122

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Valyl-tRNA ligase (valS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-21a(+)-IPP1-His

   Plasmid

   #118978

   Purpose

   Bacterial expression plasmid for Inorganic pyrophosphatase

   Depositor

   Sebastian Maerkl

   Article

   Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

   Insert

   inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
   

   Tags

   6x-His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-TetOne-Puro-LUC

   Plasmid

   #218630

   Purpose

   This plasmid contains luciferase under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LUC (LOC116160065 )
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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