We narrowed to 12,150 results for: 112

1. pYES1L-URA

   Plasmid

   #84301

   Purpose

   E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

   Depositor

   George Church

   Article

   Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLX306 iCre tetO tomato rtTA

   Plasmid

   #158050

   Purpose

   lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   tdTomato
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-Cre stuffer v3

   Plasmid

   #158030

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

   Plasmid

   #71242

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   NLS-2xMCP-2xTagRFP-T
   

   Use

   P element-mediated germline transformation

   Tags

   TagRFP-T

   Expression

   Insect

   Promoter

   Hsp83

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_502_sgCD4

   Plasmid

   #158704

   Purpose

   CD4 CRISPRa control

   Depositor

   David Root, John Doench

   Article

   Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-Q842gp160

   Plasmid

   #100931

   Purpose

   Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (Q842.d12) Env
   

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNHEJ-Cas12a-recX

   Plasmid

   #158714

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Pmyc

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQFmcs-2H12.D11

    Plasmid

    #221183

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Expression

    Bacterial

    Promoter

    PQ5 (cumate-inducible)

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-ATXN1

    Plasmid

    #48189

    Purpose

    Expresses Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-TadA8e-PcrA

    Plasmid

    #221228

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1512

    Plasmid

    #62366

    Purpose

    N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTRE2

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-VARNAM2

    Plasmid

    #195520

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST_CMV_N_Flag_BIRC6

    Plasmid

    #197973

    Purpose

    Mammalian cell expression of wt FLAG-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZJOM143

    Plasmid

    #133667

    Purpose

    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-IRES-GFP-human-Caspase-1

    Plasmid

    #183359

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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