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pscALPS-SIV-LST vpr

Plasmid

#115818

Purpose

Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVLST Vpr (AF188116)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MND1 vpr

Plasmid

#115817

Purpose

Encodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND1 Vpr (M27470)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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YFP(1-158) in pcDNAI/Amp

Plasmid

#60888

Purpose

This vector attaches YFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(1-158)

Expression

Mammalian

Mutation

Met was substituted for Gln-69.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-F63Q

Plasmid

#104363

Purpose

Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acid: F63Q

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-2/3

Plasmid

#91362

Purpose

Protein expression and purification of human SH3 domain construct NCK2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-2/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pPAtUbq10 (GB0223)

Plasmid

#68174

Purpose

Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq10 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB7

Plasmid

#19476

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB7 (DNAJB7 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0112

Plasmid

#117548

Purpose

Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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