We narrowed to 16,981 results for: tes
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Plasmid#181901PurposeFluorescently tagged HP1betaDepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only
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GFP-Tollip M240A,F241A
Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorInsertToll-Interacting Protein (TOLLIP Human)
TagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D
Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)
Plasmid#158953PurposeExpress human RAD51(T309A) mutant in mammalian cellsDepositorInsertRAD51 (RAD51 Human)
Tags2X FLAG 2X STREPExpressionMammalianMutationchanged threonine 309 to alaninePromoterCMVAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…MutationG174DPromoterPelcAAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT2res
Plasmid#106301PurposeExpresses SHMT2DepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralExpressionMammalianMutationSilent mutations destroy sgRNA targeting sitesAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
ExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MFF iso5 S155,172A
Plasmid#74383PurposeFlag tagged human S155,172A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
TagsFlagExpressionMammalianMutationSee 155 Ala, Ser 172 Ala (numbering based on MFF …Available SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX-TIR-GFP
Plasmid#52737PurposeRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and p40 Phox lipid binding domainExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-CMV-p85a 3'UTR mutant
Plasmid#51393DepositorInsertp85alpha 3'UTR (PIK3R1 Human)
UseLuciferaseTagsLuciferaseMutationmiR-29 binding site mutantPromoterCMVAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD-E12
Plasmid#25990DepositorAvailable SinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT H335G
Plasmid#13749DepositorTagsFLAGExpressionMammalianMutationChanged catalytic His 335 to GlyAvailable SinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 full
Plasmid#221401PurposeMammalian expression of human integrin beta1 full-lengthDepositorInsertintegrin beta1 full-length (ITGB1 Human)
TagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequenceAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-Rheb(S16H)
Plasmid#194204PurposeCre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.DepositorInsertRas Homolog Enriched Protein in Brain (S16H) (RHEB Human)
UseCre/LoxExpressionMammalianMutationS16HAvailable SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-WT
Plasmid#141339PurposeLentiviral vector for expression of human NHEJ1-WT in mammalian cellsDepositorInsertNHEJ1 (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianPromoterCMVAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9T210A
Plasmid#168270PurposeExpresses kinase-dead NEK9 mutant in mammalian cellsDepositorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp140-TM
Plasmid#123251PurposeMammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tetherDepositorInsertHIV-1 (DU422) Env
TagsCD5 leader peptide and Linker (with 6his tag) and…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727D-STAT3-3xFlag
Plasmid#99263PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727D)DepositorInsertSignal transducer and activator of transcription 3 - S727D (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationS727DPromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta-delta10
Plasmid#52187PurposeExpresses human ERRbeta-delta10 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only