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pT7-EGFP-C1-HsPatL1-mut2_I

Plasmid

#146549

Purpose

Mammalian Expression of HsPatL1-mut2

Depositor

Elisa Izaurralde

Publication

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-mut2 (PATL1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Expression

Mammalian

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Expression

Mammalian

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P20

Plasmid

#188055

Purpose

Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P20

Expression

Mammalian

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P30

Plasmid

#188056

Purpose

Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P30

Expression

Mammalian

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#176881

Purpose

MoClo Level 0 plasmid, contains 35S

Depositor

Daniel Voytas

Publication

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S terminator

Expression

Plant

Mutation

WT

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_70-770_J

Plasmid

#146681

Purpose

Mammalian Expression of HsPatL1_70-770

Depositor

Elisa Izaurralde

Publication

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770 (PATL1 Human)

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-epELM-5

Plasmid

#179391

Purpose

Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

Depositor

Shiro Iuchi

Publication

Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

Insert

ELMSAN1 (MIDEAS Human)

Tags

6x His

Expression

Bacterial

Promoter

T7 lac

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126A

Plasmid

#129376

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126I

Plasmid

#129377

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126I

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81I

Plasmid

#129352

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81I

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu D89A

Plasmid

#129354

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu D89A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu V90D

Plasmid

#129355

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu V90D

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81E

Plasmid

#129356

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81E

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu I88D

Plasmid

#129357

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu I88D

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pT2MPEF-NLS-QUE37C

Plasmid

#129326

Purpose

ATP measurement, nucleus, Tol2 transposon

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only
pPPC016.109

Plasmid

#171144

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJH573

Plasmid

#172157

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Untagged pylBCD); AraC

Expression

Bacterial

Mutation

Codon-optimized Methanosarcina acetivorans pylBCD

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-9251

Plasmid

#124226

Purpose

Bacterial SpCas9-HF1 expression

Depositor

Ervin Welker

Publication

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9-HF1

Use

CRISPR

Expression

Bacterial

Promoter

TetR/TetA

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPD394 VPR-ZF158

Plasmid

#138843

Purpose

Constitutive expression of VPR-ZF158 under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF158

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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