We narrowed to 8,325 results for: gnal

1. pAAV-EF1α1.1-GFP

   Plasmid

   #122100

   Purpose

   AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV2

   Insert

   GFP
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available Since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+HyPer7-NLS

   Plasmid

   #136468

   Purpose

   Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Triple nuclear localization signal of SV40 (simia…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GPRC6A-DuET

   Plasmid

   #213299

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPRC6A (GPRC6A Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTT3-SP-6xHis-NTRK1(FL)-FLAG

   Plasmid

   #157620

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NTRK1 (NTRK1 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS2M-PH-gagpol-D64V

   Plasmid

   #166031

   Purpose

   Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   MS2-Gag-Pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D64V mutation within the integrase within Pol

   Promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMDlg/pRRE-int-MS2M

   Plasmid

   #166032

   Purpose

   Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   Gag-Pol-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D64V mutation within the integrase within Pol

   Promoter

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

   Plasmid

   #141184

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Has Service

   Cloning Grade DNA

   Insert

   S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
   

   Use

   Tags

   Signal peptide from influenza HA and Superfolder …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4 HisMax-V5-GFP-RRBP1

   Plasmid

   #92150

   Purpose

   Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 (RRBP1 Human)
   

   Use

   Tags

   Calreticulin signal peptide, EGFP (A207K mutation…

   Expression

   Mammalian

   Mutation

   In RRBP1: N951S and T1329L. E1279 is also missing.

   Promoter

   CMV

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CCR2-DuET

   Plasmid

   #213198

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR2 (CCR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEP4-myc-ACE2

    Plasmid

    #141185

    Purpose

    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-FLAG-RGS14

    Plasmid

    #236567

    Purpose

    Expresses Flag-RGS14 in mammalian cells

    Depositor

    John Hepler

    Article

    Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

    Insert

    Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eGFP-proα2(I)

    Plasmid

    #119826

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGF-GAS4X

    Plasmid

    #225751

    Purpose

    This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

    Depositor

    Eun Hee Han

    Article

    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

    Insert

    interferon gamma activating sequence (GAS) (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-αIFNα-ab

    Plasmid

    #110799

    Purpose

    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells

    Depositor

    Thomas Böldicke

    Article

    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

    Insert

    antibody against mouse interferon alpha
    

    Use

    Tags

    ER signal peptide, His6 tag, and myc epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid

    #141183

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CXCR3-DuET

    Plasmid

    #213220

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPR63-DuET

    Plasmid

    #213289

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR63 (GPR63 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA GASTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71295

    Purpose

    Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GASTM mutated glycosylation motif, HA, Lamp2 sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CXCR7-DuET

    Plasmid

    #213224

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR7 (ACKR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge