We narrowed to 19,582 results for: Cre

1. pCAG-uMASS_1

   Plasmid

   #209762

   Purpose

   Expression of uMASS_1 in mammalian cells under CAG promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_1
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. NylB'-SCY_pET21b

   Plasmid

   #215417

   Purpose

   Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB'-SCY
   

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ST-SBP-mCherry_puromycin

   Plasmid

   #65271

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG306-GPD-VMA1 chr I

   Plasmid

   #41896

   Purpose

   Yeast expression of VMA1.

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Insert

   VMA1 (VMA1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Contains silent mutation at 342A-G and L87S mutat…

   Promoter

   GPD

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. actc1b-mKate2-ING2

   Plasmid

   #109502

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-ING2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI pHluorin Syt11

   Plasmid

   #184512

   Purpose

   Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 11 (Syt11 Mouse)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7-K122-TEAD-p-H2B-mCherry

   Plasmid

   #139648

   Purpose

   Reporter assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   IRES-GFP
   

   Use

   Lentiviral

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-BFP

   Plasmid

   #106282

   Purpose

   A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBO.ACT003

    Plasmid

    #182713

    Purpose

    aTc inducible dCasRx-IF3

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 3

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-LOx-IRES-EGFP

    Plasmid

    #166877

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-6020nes

    Plasmid

    #108656

    Purpose

    A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-JNK-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX Vav1 Y3F uGFP

    Plasmid

    #14558

    Depositor

    Joan Brugge

    Article

    Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

    Insert

    Vav1 (VAV1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y3F (Y142, Y160, Y174) in DH domain

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pGWB450

    Plasmid

    #74832

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-PAK2 wt

    Plasmid

    #31671

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGWB416

    Plasmid

    #74810

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDR111_Bgal NLS

    Plasmid

    #188396

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Promoter

    Phyper spank

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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