We narrowed to 9,087 results for: sgRNA

Showing: 7861 - 7880 of 9087 results

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Publication

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Publication

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Publication

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM L368G

Plasmid

#169985

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, L368G

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM R394E

Plasmid

#169987

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, R394E

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F405G

Plasmid

#169988

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F405G

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM F370G

Plasmid

#169986

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, F370G

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM L368G

Plasmid

#169978

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, L368G

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM R394E

Plasmid

#169980

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, R394E

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM S297A

Plasmid

#169976

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, S297A

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F370G

Plasmid

#169979

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, F370G

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F405G

Plasmid

#169981

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, F405G

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Bh

Plasmid

#136378

Purpose

Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1-2

Plasmid

#124918

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCas15

Plasmid

#82383

Purpose

sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas14

Plasmid

#82382

Purpose

sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas13

Plasmid

#82381

Purpose

sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGH020 sgZmat3.1

Plasmid

#227943

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Publication

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-C

Plasmid

#220899

Purpose

AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-C

Use

AAV

Expression

Mammalian

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-V2-Puro.P2A.mCherry

Plasmid

#242847

Purpose

Backbone for cloning U6-driven sgRNAs

Depositor

Siyuan Zhang

Publication

Aleksandrovic et al Nat Commun. 2026 Sep 3;17(1):10497. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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