We narrowed to 8,688 results for: App

1. mEos3.2-CCPB1-N-18

   Plasmid

   #57449

   Purpose

   Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   CCPB1
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. tdEos-CCPB1-C-18

   Plasmid

   #57591

   Purpose

   Localization: Actin Capping Protein , Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Insert

   CCPB1
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

   Plasmid

   #86451

   Purpose

   Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)

   Depositor

   Gregor Bucher

   Insert

   Hsp_p-GAL4Delta-SV40;attp
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSET.HaloTag.v7.AviTag

   Plasmid

   #195486

   Purpose

   Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7.AviTag
   

   Use

   Tags

   6xHIS and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR ARRET

   Plasmid

   #111384

   Purpose

   ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

   Depositor

   Klaus Holzmann

   Insert

   ARRET
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PS-CFP2-MCPB2-N-18

   Plasmid

   #57239

   Purpose

   Localization: Mannosidase/Golgi, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   MCPB2
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pET12a-AL-103 delProIns

   Plasmid

   #47095

   Depositor

   Marina Ramirez-Alvarado

   Insert

   AL-103 delProIns (IGKV3-20 Human)
   

   Use

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   delete aa95

   Promoter

   T7

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-C1-NuMA

   Plasmid

   #81029

   Purpose

   Expresses human NuMA with N-terminal EGFP tag in mammalian cells

   Depositor

   Michael Mancini

   Insert

   Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AbVec_IGKC

   Plasmid

   #183702

   Purpose

   Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

   Depositor

   David Nemazee

   Insert

   IGKC (IGKC )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOpen-BstpolLF

    Plasmid

    #165553

    Purpose

    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Bst DNA Polymerase, Large Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pEYFP-C1-NuMA

    Plasmid

    #28238

    Purpose

    Expresses human NuMA with N-terminal EYFP tag in mammalian cells

    Depositor

    Michael Mancini

    Insert

    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK637

    Plasmid

    #72437

    Purpose

    Acetobacter aceti 1023 mutS, partial gene

    Depositor

    T. J. Kappock

    Insert

    DNA mismatch repair protein MutS (partial gene)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletes nt 1-767 and nt 2086-2655 of mutS gene

    Promoter

    n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXR003_puroR

    Plasmid

    #219819

    Purpose

    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV hH1-ARRET

    Plasmid

    #111374

    Purpose

    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Insert

    ARRET w/o polyA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV UbC6-ARRET

    Plasmid

    #111378

    Purpose

    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Insert

    ARRET polyA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbC6

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXR004_puroR

    Plasmid

    #219820

    Purpose

    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CAG-K-SPOTIT

    Plasmid

    #191488

    Purpose

    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-SPOTIT
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEW-pHluorin2-PTS1

    Plasmid

    #213776

    Purpose

    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei

    Depositor

    Kenneth Christensen

    Insert

    pHlorin2
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Appended a 2 glycine linker and a T. brucei perox…

    Promoter

    T. brucei rRNA promotor

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge