We narrowed to 8,596 results for: Set

1. pME-lox-3xSTOP-lox (JDW 869)

   Plasmid

   #224521

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   LoxP-3xStop-LoxP
   

   Use

   Cre/Lox

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-[EF1α-CD19 4-1BB]

   Plasmid

   #218336

   Purpose

   Lentiviral transfer plasmids for mammalian expression CD19 CAR

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-[EF1α-CD19 CD28Z]

   Plasmid

   #218338

   Purpose

   Lentiviral transfer plasmids for mammalian expression CD19 CAR

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Puro-sTagRFP-TUBA1B

   Plasmid

   #207769

   Purpose

   Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLVX-TetOne-Puro-PFDN2

   Plasmid

   #202664

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   prefoldin subunit 2 (PFDN2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-DMD-Donor

   Plasmid

   #60605

   Purpose

   Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   Hygromycin resistant gene
   

   Use

   Knock-in donor vector

   Expression

   Mammalian

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. SOX17-NLS-tdTomato-EPG

   Plasmid

   #210466

   Purpose

   Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

   Plasmid

   #154775

   Purpose

   plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAA in GFP reporter, UUA anticodon and G10A, …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB53-Pac-TRE-3xFlag-Neurog1-attB53

   Plasmid

   #183613

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Neurog1 (Neurog1 Mouse)
   

   Use

   Rmce

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCol1a1-frt (FVB)

    Plasmid

    #63575

    Purpose

    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting; Frt/flpe

    Expression

    Bacterial and Mammalian

    Promoter

    None

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC-Flag-CSNK1A1 G40N-Puro

    Plasmid

    #123320

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Glycine 40 to Asparagine

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBA57-TEV-His12

    Plasmid

    #137033

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length BBA57, contains signal pep…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSHUT4-eYFP

    Plasmid

    #133094

    Purpose

    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

    Depositor

    Jens Laurids Sørensen

    Article

    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

    Insert

    enhanced yellow flourescent protein
    

    Use

    Fungal expression

    Promoter

    TEF-1α

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

    Plasmid

    #154776

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in GFP reporter, hybrid PylT with A…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDragon-Ctgf

    Plasmid

    #155015

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Ccn2 (Ccn2 Mouse)
    

    Use

    Mouse Targeting

    Mutation

    M24T (please see depositor comments)

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

    Plasmid

    #179415

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.Chromo/TAF3.PHD-13XL-BASU
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xhyb PylT A41AA C55A sfGFP 150TAG

    Plasmid

    #154772

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, hybrid PylT with A41AA an…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid

    #178224

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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