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We narrowed to 16,181 results for: nes

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Showing: 7961 - 7980 of 16181 results

  2. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid
    #183606
    Purpose
    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    HA, Human PDGFRB transmembrane domain, Mouse IgGK…
    Expression
    Mammalian
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183606-plasmid-map-sequence-id-361592

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  4. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161817-plasmid-map-sequence-id-320651

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  6. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Publication
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

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  7. pDEST-ERBB2-v1

    Plasmid
    #154901
    Purpose
    Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays
    Depositor
    Johanna Ivaska
    Publication
    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERBB2 (ERBB2 Human)
    Tags
    Venus fragment 1 (v1)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154901-plasmid-map-sequence-id-306904

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  9. Calb1-T2A-dgCre targeting vector

    Plasmid
    #114438
    Purpose
    Target dgCre into the endogenous mouse Calb1 locus at the stop codon
    Depositor
    Hongkui Zeng
    Publication
    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dgCre
    Use
    Mouse Targeting
    Tags
    DHFR domain and EGFP
    Promoter
    none; utilizes endogenous Calb1 promoter for expr…
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    114438-plasmid-map-sequence-id-227320

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  12. pCMV-hA3A-BE3-Y132D

    Plasmid
    #113425
    Purpose
    Expresses hA3A-BE3-Y132D in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-BE3-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y132D
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    113425-plasmid-map-sequence-id-217957

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  13. pCMV-hA1-BE3

    Plasmid
    #113418
    Purpose
    Expresses hA1-BE3 in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    113418-plasmid-map-sequence-id-217359

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  16. B52 + SPRTN sgSTOP

    Plasmid
    #100709
    Purpose
    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting SPRTN (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100709-plasmid-map-sequence-id-193207

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  19. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pME-hygro-hPGAP3-3HA

    Plasmid
    #50373
    Purpose
    Express C-terminally triple HA tagged human PGAP3 in mammmalian cells
    Depositor
    Reika Watanabe
    Publication
    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
    Tags
    3x HA
    Expression
    Mammalian
    Promoter
    SR alpha
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7961 - 7980 of 16181 results