We narrowed to 23,988 results for: Nes

1. pEND-400_Cacnes_CAMP2

   Plasmid

   #225606

   Purpose

   Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

   Depositor

   Marc Güell

   Article

   Guillermo et al Cell Systems, 2025

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEND-418_Cacnes_LysA

   Plasmid

   #225607

   Purpose

   Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

   Depositor

   Marc Güell

   Article

   Guillermo et al Cell Systems, 2025

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

   Plasmid

   #100857

   Purpose

   AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-Kinesin11-N-18

   Plasmid

   #55067

   Purpose

   Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Kinesin11 (KIF11 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCardinal-Farnesyl-5

   Plasmid

   #56159

   Purpose

   Localization: Plasma Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pmScarlet-H_NES_C1

   Plasmid

   #85061

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-4511NES

   Plasmid

   #138375

   Purpose

   Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Booster-ERK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-Farnesyl-5

   Plasmid

   #55045

   Purpose

   Localization: Plasma Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV_NESPylRS(WT)_hU6tRNAPyl

   Plasmid

   #223509

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6 and U6

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-4493NES

    Plasmid

    #138373

    Purpose

    Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEND-184_Cacnes_mCherry

    Plasmid

    #225610

    Purpose

    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNES-iRFP-C1

    Plasmid

    #116863

    Purpose

    cytosolic iRFP

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    iRFP713
    

    Use

    Tags

    X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEND-229_Cacnes_EforCP

    Plasmid

    #225615

    Purpose

    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    EforCP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid

    #225617

    Purpose

    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEmerald-Kinesin11-N-18

    Plasmid

    #54137

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEND-303_Cacnes_tetR

    Plasmid

    #225618

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEND-304_Cacnes_tetR

    Plasmid

    #225619

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEND-375_Cacnes_phlF

    Plasmid

    #225620

    Purpose

    C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    PhlF
    

    Use

    Synthetic Biology

    Tags

    6xHis and mCherry-V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEND-192_Cacnes_HS

    Plasmid

    #225621

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEND-226_Cacnes_mCitrine

    Plasmid

    #225613

    Purpose

    Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Insert

    mCitrine
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEmerald-Farnesyl-5

    Plasmid

    #54093

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100849

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-4580NES

    Plasmid

    #138376

    Purpose

    Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-JNK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100850

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS-4591NES

    Plasmid

    #138377

    Purpose

    Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ROCK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNES-VL12.3

    Plasmid

    #43862

    Depositor

    Dane Wittrup

    Article

    Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

    Insert

    VL12.3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

30. mRuby2-Farnesyl-5

    Plasmid

    #55896

    Purpose

    Localization: Membrane, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge