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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,988 results for: Nes

Showing: 151 - 200 of 23988 results

  1. mApple-Farnesyl-5

    Plasmid
    #54899
    Purpose
    Localization: Plasma Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. mTurquoise-Farnesyl-5

    Plasmid
    #55551
    Purpose
    Localization: CAAX - Membrane, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. nes1852tk/lacZ

    Plasmid
    #47613
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.
    Insert
    nestin 2nd intron (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    2nd intron only
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. mRuby-Farnesyl-5

    Plasmid
    #55861
    Purpose
    Localization: Membrane, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. PA-mCherry1-Farnesyl-5

    Plasmid
    #57176
    Purpose
    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    57176-plasmid-map-sequence-id-209017

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  9. mCerulean-Farnesyl-5

    Plasmid
    #55367
    Purpose
    Localization: Membrane, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55367-plasmid-map-sequence-id-183116

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  11. nes374tk/lacZ

    Plasmid
    #47615
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    374 bp from 2nd intron (comprising bp 1153 to 152…
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. mKO-Farnesyl-5

    Plasmid
    #57841
    Purpose
    Localization: Plasma Membrane, Excitation: 548, Emission: 561
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mKO
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. iLOV-Farnesyl-5

    Plasmid
    #56516
    Purpose
    Localization: Membrane, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. HA-CPI-17-wt-NES

    Plasmid
    #100775
    Purpose
    Mammalian expression of CPI-17 cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-NES (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100775-plasmid-map-sequence-id-193158

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  17. mKO2-Farnesyl-5

    Plasmid
    #57904
    Purpose
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mKO2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

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  20. pEND-350_Cacnes_ori

    Plasmid
    #225605
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225605-plasmid-map-sequence-id-451372

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  21. pEND-443_Cacnes_HisB

    Plasmid
    #225608
    Purpose
    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225608-plasmid-map-sequence-id-451366

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  22. pEND-446_Cacnes_ProA

    Plasmid
    #225609
    Purpose
    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225609-plasmid-map-sequence-id-451370

    Enlarge

  23. pEND-224_Cacnes_sfGFP

    Plasmid
    #225611
    Purpose
    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225611-plasmid-map-sequence-id-451364

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  24. pEND-225_Cacnes_mKO2

    Plasmid
    #225612
    Purpose
    Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mKO2
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225612-plasmid-map-sequence-id-451369

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  27. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  29. L_NES 80kDa

    Plasmid
    #201390
    Purpose
    Nucleocytoplasmic transport probe
    Depositor
    Pere Roca-Cusachs
    Insert
    Adeno_NES-EGFP-8PrA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201390-plasmid-map-sequence-id-400570

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  30. M_NES 80kDa

    Plasmid
    #201388
    Purpose
    Nucleocytoplasmic transport probe
    Depositor
    Pere Roca-Cusachs
    Insert
    MAPK_NES-EGFP-8PrA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201388-plasmid-map-sequence-id-399888

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  32. H_NES 80kDa

    Plasmid
    #201389
    Purpose
    Nucleocytoplasmic transport probe
    Depositor
    Pere Roca-Cusachs
    Insert
    HIV_NES-EGFP-8PrA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201389-plasmid-map-sequence-id-399915

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Showing: 151 - 200 of 23988 results