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pBR HPV31 9E

Plasmid

#221009

Purpose

Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

Depositor

Alison McBride

Insert

Human papillomavirus type 31 genome 9E isolate

Use

Cloning vector

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClpP-his

Plasmid

#22144

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

ClpP-His6

Tags

his

Expression

Bacterial

Mutation

C-terminal His tag

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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Cd2ap-GFP

Plasmid

#221528

Purpose

Fluorescently tagged Cd2ap-GFP

Depositor

Alessandro Ori

Insert

Cd2ap (CD2AP Human)

Tags

GFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

Plasmid

#221365

Purpose

Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Insert

Anti-Rho IPI-[1D4] Mouse/Rabbit HC

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

Plasmid

#221366

Purpose

Mammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Insert

Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCSC-NLS-RFP

Plasmid

#221813

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NLS-mCherry

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(H840A)-T2A-EGFP

Plasmid

#221231

Purpose

Express nCas9 nickase (H840A) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(H840A)-T2A-EGFP

Expression

Mammalian

Mutation

H840A

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-tadDE-PcrA M6-UGI

Plasmid

#221236

Purpose

Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

tadDE-PcrA M6-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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SMN2E7-Fluc-Nluc-reporter

Plasmid

#221005

Purpose

Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

Fluc-SMN2E7-STOP-Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSC-NLS-EGFP

Plasmid

#221811

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NLS-EGFP

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDP0437

Plasmid

#221127

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

pHluorin

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

Plasmid

#221234

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA

Plasmid

#221228

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-OPHN1

Plasmid

#221310

Purpose

Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

OPHN1 (pLxIS) (Ophn1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CAMK2D

Plasmid

#221687

Purpose

Mammalian expression of FLAG-tagged human CAMK2D

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Expression

Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-BLM

Plasmid

#221226

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-BLM

Expression

Mammalian

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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