We narrowed to 107 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Publication

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1761 Articles

   

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These results may also be relevant.

1. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Publication

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AP1muA single guide RNA

   Plasmid

   #230030

   Purpose

   guide RNA for AP1muA tagging at the C-terminus in pX459

   Depositor

   Francesca Bottanelli

   Publication

   Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   AP1M1 guide (AP1M1 Human)
   

   Use

   CRISPR

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1 Article

   

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3. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Publication

   Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-CTNNA1(α-catenin CRISPR KO)

   Plasmid

   #229708

   Purpose

   Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

   Depositor

   Alpha Yap

   Publication

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX459v2-ILK-gRNA 1-exon 1

   Plasmid

   #163320

   Purpose

   SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

   Depositor

   Val Brunton

   Publication

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   ILK gRNA 1_Exon1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

6. px459-Rheb sgRNA

   Plasmid

   #133768

   Purpose

   Expresses Cas9 and human Rheb sgRNA

   Depositor

   Shawn Ferguson

   Publication

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   Rheb sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124288

   Purpose

   sgRNA against human NFATc3

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   NFATc3 nuclear factor of activated T cells (NFATC3 Human)
   

   Use

   CRISPR

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

8. PX459v2-ILK-gRNA 2-exon 8

   Plasmid

   #163321

   Purpose

   SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

   Depositor

   Val Brunton

   Publication

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   ILK gRNA 2_Exon8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6F

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

9. px459-RhebL1 sgRNA

   Plasmid

   #133769

   Purpose

   Expresses Cas9 and human RhebL1 sgRNA

   Depositor

   Shawn Ferguson

   Publication

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Has service

   50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

   Insert

   RhebL1 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCP

    Plasmid

    #204743

    Purpose

    Cas9-2A-Puro expression vector

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPH3-GS3

    Plasmid

    #204761

    Purpose

    Expression of Cas9 and human H3

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H3 (H3 Human)
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPH3.3-GS3

    Plasmid

    #204764

    Purpose

    Expression of Cas9 and human H3.3

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H3.3
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPH3.2-GS3

    Plasmid

    #204763

    Purpose

    Expression of Cas9 and human H3.2

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H3.2 (HIST2H3PS2 Human)
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCPH2A.J-GS3

    Plasmid

    #204757

    Purpose

    Expression of Cas9 and human H2A.J

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AJ (H2AJ Human)
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPH2A.B-GS3

    Plasmid

    #204760

    Purpose

    Expression of Cas9 and human H2A.B

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AB1 (H2AFB1 Human)
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX459_NRF2-exon5-1

    Plasmid

    #177781

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

    Depositor

    Michael Ristow

    Publication

    Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    CRISPR

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX459_NRF2-exon5-2

    Plasmid

    #177782

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

    Depositor

    Michael Ristow

    Publication

    Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    CRISPR

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCPH2A.L-GS3

    Plasmid

    #204756

    Purpose

    Expression of Cas9 and human H2A.L

    Depositor

    Yuichiro Miyaoka

    Publication

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AL3 (H2AC6 Human)
    

    Expression

    Mammalian

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX459_GRHL1-exon5

    Plasmid

    #177763

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Publication

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459_GRHL1-exon8

    Plasmid

    #177764

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Publication

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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