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Plasmid#202553PurposeNontargeting control vector with TagBFP2DepositorInsertHumanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPuromycin-resistance gene in the pLV hU6-sgRNA hU…PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)
Plasmid#202554PurposeSynaptotagmin-1 sgRNA1 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCambia-PUP-IT
Plasmid#186478PurposeExpresses POI-PUP-IT fusion in PlantsDepositorInsertFLAG-Pup(E)
UseTagsTriple Flag TagExpressionPlantMutationPromoter35SAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(G164)
Plasmid#165994PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(G164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A149)
Plasmid#165998PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A149)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(E167)
Plasmid#166004PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(E167)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(D165)
Plasmid#166003PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(D165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(C164)
Plasmid#166002PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(C164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(N180)
Plasmid#166000PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A165)
Plasmid#165999PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-HisDAD2 cPGFP(G164)
Plasmid#165997PurposeExpression of coding sequence in Escherichia coliDepositorInsertHisDAD2 cPGFP(G164)
UseTags6HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-HisDAD2
Plasmid#165996PurposeExpression of coding sequence in Escherichia coliDepositorInsertHisDAD2
UseTags6HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(L166)
Plasmid#165995PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(L166)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(G164)
Plasmid#165993PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(G164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneUseTagsGal4 DNA binding domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p255 pPr-CREM/Ins/CMV-STOP-luc
Plasmid#8408PurposeModified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.DepositorInsertCREM/insulators/lox/luciferase
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterProbasin, CMVAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
p252 pPr-CREM/CMV-STOP-luc
Plasmid#8406PurposeExpression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.DepositorInsertCREM/lox/luciferase
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterProbasin promoter, CMVAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyS
Plasmid#181728PurposeGalactose inducible expression of 3xFLAG-PolySDepositorInsert3xFLAG-PolyS
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only