We narrowed to 8,304 results for: FIE

1. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

   Plasmid

   #202554

   Purpose

   Synaptotagmin-1 sgRNA1 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCambia-PUP-IT

   Plasmid

   #186478

   Purpose

   Expresses POI-PUP-IT fusion in Plants

   Depositor

   Jen Sheen

   Article

   Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

   Insert

   FLAG-Pup(E)
   

   Use

   Tags

   Triple Flag Tag

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19b-rDAD2 cPGFP(G164)

   Plasmid

   #165994

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(G164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET19b-DAD2 cPGFP(A149)

   Plasmid

   #165998

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(A149)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET19b-rShHTL7 cPGFP(E167)

   Plasmid

   #166004

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(E167)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET19b-rShHTL7 cPGFP(D165)

   Plasmid

   #166003

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(D165)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET19b-rShHTL7 cPGFP(C164)

   Plasmid

   #166002

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(C164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET19b-DAD2 cPGFP(N180)

   Plasmid

   #166000

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(N180)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET19b-DAD2 cPGFP(A165)

    Plasmid

    #165999

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A165)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET19b-HisDAD2 cPGFP(G164)

    Plasmid

    #165997

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2 cPGFP(G164)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET19b-HisDAD2

    Plasmid

    #165996

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET19b-rShHTL7 cPGFP(L166)

    Plasmid

    #165995

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(L166)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET19b-DAD2 cPGFP(G164)

    Plasmid

    #165993

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(G164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOAD

    Plasmid

    #196825

    Purpose

    Yeast two-hybrid ; Gal4 activation domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOBD2

    Plasmid

    #196826

    Purpose

    Yeast two-hybrid ; Gal4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p255 pPr-CREM/Ins/CMV-STOP-luc

    Plasmid

    #8408

    Purpose

    Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/insulators/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin, CMV

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

19. p252 pPr-CREM/CMV-STOP-luc

    Plasmid

    #8406

    Purpose

    Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin promoter, CMV

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pAG426-GAL-3xFLAG-PolyS

    Plasmid

    #181728

    Purpose

    Galactose inducible expression of 3xFLAG-PolyS

    Depositor

    Alejandro Chavez

    Insert

    3xFLAG-PolyS
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge