We narrowed to 119 results for: HBB
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Plasmid#49969PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only
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JA1272
Plasmid#49967PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1266
Plasmid#49966PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1229
Plasmid#49955PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1217
Plasmid#49954PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
modified pcAT7-Glo1
Plasmid#160996PurposeSplicing reporter backboneDepositorInserthemoglobin subunit beta (HBB Human)
ExpressionMammalianMutationRepeated intron 1 with 40 nucelotide deletionPromoterCAGAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)
Plasmid#189793PurposeExpress wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)
Plasmid#189791PurposeExpress wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)
Plasmid#189792PurposeExpress wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)
Plasmid#189794PurposeExpress GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-BGG-PTC_E
Plasmid#146191PurposeMammalian Expression of BGG-PTCDepositorInsertBGG-PTC (HBB Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-BGG-WT_E
Plasmid#146192PurposeMammalian Expression of BGG-WTDepositorInsertBGG-WT (HBB Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
Plasmid#189795PurposeExpress GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPonA-BI-Gl NORM-LacZA TER- LacZB
Plasmid#86212Purposebeta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoterDepositorUsePonasterone a inducible promoterTagslacZA or lacZBExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterPonasterone A inducibleAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB
Plasmid#86194Purposebeta Globin NMD construct with and without PTC under Bi-directional TET on promoterDepositorUseTet onExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterTet onAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAX198
Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-Terminal Split Cas9 with GyrA intein
Plasmid#58694PurposeExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
UseAAV and CRISPRTagsGyrA Csplit Intein and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xms2-2x3’UTR
Plasmid#122946PurposeFor expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-MS2-HBB 3' UTR
UseAAVExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xPP7-2x3'UTR
Plasmid#122947PurposeFor expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-PP7-HBB 3' UTR
UseAAVExpressionMammalianAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only