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pGL3-Basic-Rab18-sfGFP(N) HDR template

Plasmid

#129415

Purpose

HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

RAB18 HDR template (RAB18 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X2-hDrp1(518-736)-Flag

Plasmid

#37244

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Drp1 (DNM1L Human)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

contains AA 517-end

Promoter

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

Plasmid

#110735

Purpose

Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

H2B-mOrange2

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

Plasmid

#215759

Purpose

HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

Depositor

Dimitrios Wagner

Article

Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

Insert

CD247-truncCD19CAR-GSG (CD19, CD247 Human)

Use

Tags

IgG1 CH3

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

Plasmid

#215769

Purpose

encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

Depositor

Dimitrios Wagner

Article

Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

Inserts

TRAC-5' homology arm (TRAC Human)
P2A-CD19.28.zeta-bgH (CD19 Mouse, Bovine, Human)
TRAC-3' homology arm (TRAC Human)

Use

Tags

Expression

Bacterial

Mutation

Cas12a PAM mutated

Promoter

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-CD3z-truncCAR(anti-CD19)

Plasmid

#215758

Purpose

HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

Depositor

Dimitrios Wagner

Article

Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

Insert

CD247-truncCD19CAR (CD247, CD19 Human)

Use

Tags

IgG1 CH3

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge