Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 94 results for: OFP

Clear keywords
Showing: 61 - 80 of 94 results

  1. pKT-PDGFRa-IRES-Katushka

    Plasmid
    #133081
    Purpose
    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFRa (Pdgfra Mouse)
    Expression
    Mammalian
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    133081-plasmid-map-sequence-id-515604

    Enlarge

  2. AiRTi

    Plasmid
    #214068
    Purpose
    Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Linker sequence present between RFP and GFP
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214068-plasmid-map-sequence-id-423979

    Enlarge

  3. ART_RTctrl

    Plasmid
    #214066
    Purpose
    Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Linker sequence present between RFP and GFP
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214066-plasmid-map-sequence-id-423981

    Enlarge

  5. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Publication
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67277-plasmid-map-sequence-id-121189

    Enlarge

  6. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Publication
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172473-plasmid-map-sequence-id-342817

    Enlarge

  7. AiRT

    Plasmid
    #214067
    Purpose
    Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Linker sequence present between RFP and GFP
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214067-plasmid-map-sequence-id-423798

    Enlarge

  10. pKT-H3.3-K27M-katushka

    Plasmid
    #209443
    Purpose
    SB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)
    Depositor
    Maria Castro
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H3.3-K27M
    Expression
    Mammalian
    Mutation
    K27M
    Available since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    209443-plasmid-map-sequence-id-418361

    Enlarge

  11. AiRTX_ctrl

    Plasmid
    #214069
    Purpose
    Fluorescent reporter system, NMD negative control. Intron present in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Remnant of linker sequence present at the end of …
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214069-plasmid-map-sequence-id-423978

    Enlarge

  14. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

    Enlarge

  16. AiRT_RTctrl

    Plasmid
    #214070
    Purpose
    Fluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR
    Depositor
    Sujatha Jagannathan
    Publication
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboRFP
    Expression
    Mammalian
    Mutation
    Linker sequence present between RFP and GFP
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214070-plasmid-map-sequence-id-423656

    Enlarge

  17. pMLS791

    Plasmid
    #188889
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Depositor
    Erik Jorgensen
    Publication
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188889-plasmid-map-sequence-id-370713

    Enlarge

Showing: 61 - 80 of 94 results