We narrowed to 9,122 results for: Pol

1. Cas9_P2A_T4pol

   Plasmid

   #190146

   Purpose

   Expresses SpCas9 and T4pol

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_T4pol
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9_P2A_POLB

   Plasmid

   #190145

   Purpose

   Expresses SpCas9 and POLB

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9_P2A_POLB
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPOLY_new

   Plasmid

   #179276

   Purpose

   Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLz58_polyPro_CsoS2_CTP

   Plasmid

   #140850

   Purpose

   Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 C-terminal peptide
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz55_polyPro_random_Npeptide

   Plasmid

   #140846

   Purpose

   Same as pLz47 but with randomized N*-peptide sequence

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with randomized CsoS2 N-peptide
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz56_polyPro_CsoS2_MR_r1

   Plasmid

   #140848

   Purpose

   Same as pLz47 but with MR repeat 1 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 MR repeat 1
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLz47_polyPro_CsoS2_Npeptide

   Plasmid

   #140845

   Purpose

   Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 N-peptide
   

   Tags

   Hexahistidine tag and Strep-tag II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz57_polyPro_CsoS2_CTD_r8

   Plasmid

   #140849

   Purpose

   Same as pLz47 but with CTD repeat 8 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 CTD repeat 8
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PoliIFN-γ

   Plasmid

   #107289

   Purpose

   Plasmid can be used for secretion of fish Interferon gamma

   Depositor

   Bohdan Schneider

   Article

   Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

   Insert

   Interferon gamma
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLJM1 polylinker

    Plasmid

    #164143

    Purpose

    Lentiviral expression vector pLJM1 with extended polylinker

    Depositor

    Richard Possemato

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-GBDpol

    Plasmid

    #165501

    Purpose

    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Pyrococcus Sp. Heat-Stable DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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