Skip to main content
Addgene
Showing: 61 - 80 of 14496 results

  3. pET-Cb2

    Plasmid
    #53529
    Purpose
    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)
    Insert
    mitochondrial arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    changed Ser4 to Ala4 to permit NcoI cloning
    Promoter
    T7
    Available since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    53529-plasmid-map-sequence-id-83364

    Enlarge

  4. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

    Enlarge

  8. pET-sCS4T

    Plasmid
    #28249
    Depositor
    Yawen Bai
    Article
    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
    Insert
    single chain Cse4-Scm3-H4 with Thrombin site
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A thrombin cutting site between Cse4 and Scm3
    Promoter
    Available since
    July 12, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. pET-sC4T

    Plasmid
    #28251
    Depositor
    Yawen Bai
    Article
    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
    Insert
    single chain Cse4-H4 with Thrombin site
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A thrombin cutting site between Cse4 and H4
    Promoter
    Available since
    July 11, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. pET28b-XI

    Plasmid
    #157988
    Purpose
    Expresses XI in E.coli cell
    Depositor
    Byung Il Lee
    Insert
    xylose isomerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    157988-plasmid-map-sequence-id-309115

    Enlarge

  17. pET_BGL3

    Plasmid
    #101912
    Purpose
    Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.
    Depositor
    Steve Shih
    Article
    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7+LacO
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    101912-plasmid-map-sequence-id-198935

    Enlarge

  18. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

    Enlarge

  20. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

    Enlarge

Showing: 61 - 80 of 14496 results