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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NmCas9-FLAG

Plasmid

#47872

Purpose

FLAG tagged NmCas9 with no NLS.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11A

Plasmid

#239212

Purpose

Expresses CDK11A in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11A (CDK11A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Neo-CMV>hCCNL2

Plasmid

#239219

Purpose

Expresses CCNL2 in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin L2 (CCNL2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11B

Plasmid

#239211

Purpose

Expresses CDK11B in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11B (CDK11B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11A G567S

Plasmid

#239214

Purpose

Expresses CDK11A with G567S resistance mutation in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11A with G567S resistance mutation (CDK11A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 567 to Serine

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_WDR54:1-334

Plasmid

#210931

Purpose

Insect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR54:M1-V334 (WDR54 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-C_TLE1:1-770

Plasmid

#211322

Purpose

Mammalian expression of full-length human TLE1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TLE1:M1-Y770 (TLE1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR55:1-383

Plasmid

#211041

Purpose

Mammalian expression of full-length human WDR55. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR55:M1-D383 (WDR55 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

C151R natural variant (VAR_037057)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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