We narrowed to 16,118 results for: form
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Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pESUMO-hRORg1
Plasmid#170330PurposeExpresses RORg isoform 1 with N-terminal HisSUMO tag in bacteriaDepositorAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHC039 (∆phcA)
Plasmid#226207PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
GST-beta-arrestin1
Plasmid#36918DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 A50I mutation
Plasmid#46271DepositorInsertVcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
TagsEGFPExpressionMammalianMutation1-258 (VD1 domain) A50I mutationPromoterCMVAvailable SinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 A50I mutant
Plasmid#46269DepositorInsertVcl 1-851 A50I mutation (VCL Chicken)
TagsEGFPExpressionMammalianMutation1-851 (Vh) head domain, A50I mutationPromoterCMVAvailable SinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMHBAD14
Plasmid#42304DepositorInsertNpuDnaE C-intein
TagsGB1-H6ExpressionBacterialPromoteraraBADAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg2
Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
TagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225645PurposetetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA and LacO3O1 promotersAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225648PurposeLacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
TagsmCherryExpressionBacterialPromoterLacO3O1 and tetA promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225647PurposeLacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInsertLacO3O1 and tetR/tetA in tandem conformation
TagsmCherryExpressionBacterialPromoterLacO3O1 and tetA promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225646PurposetetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421)
Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
TagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
TagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…Available SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
TagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361AAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2A-2A 2B
Plasmid#213626PurposeBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2BDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB-2A
Plasmid#213627PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB-2ADepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1 5W
Plasmid#213629PurposeBacterial expression of SUMO-tagged rat FL-syt1 5WDepositorInsertFL-syt1 5W (Syt1 Rat)
TagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-Ce25
Plasmid#51296PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
TagsHis Tag and ThioredoxinExpressionBacterialMutationGTT (Val) insertion after initiation codon to per…PromoterT7Available SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-851 A50I mutation
Plasmid#46174DepositorInsertVcl 1-851 A50I (VCL Chicken)
TagsHisExpressionBacterialMutationaa 1-851 A50I mutation (talin binding domain mut…PromoterT7Available SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 WT1 +1 ATG
Plasmid#217643PurposeDox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)DepositorInsertWT1 long isoform (WT1 Human)
ExpressionMammalianAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC024 (∆SH)
Plasmid#226202PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).DepositorInsertHomology arms for soluble hydrogenase operon
UseSynthetic BiologyMutation∆hoxFUYHWI ∆hypA2B2F2Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC022 (∆phcA)
Plasmid#226200PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC004 (∆phaCAB)
Plasmid#226198PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 15, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA, araBAD and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 WT1 +378 ATG
Plasmid#217644PurposeDox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)DepositorInsertWT1 short isoform (WT1 Human)
ExpressionMammalianAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
TagsSUMOExpressionBacterialMutationY96C, C139A, C141SAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-codA
Plasmid#190058PurposeExpress the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertcodA
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2000
Plasmid#101197PurposeName: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.DepositorInsertLANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
UseLuciferaseAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-HA
Plasmid#49851PurposeEncodes human connexin 43 with a C-terminal HA epitope fusion tagDepositorInsertConnexin 43 (GJA1 Human)
TagsHAExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mKate2
Plasmid#162624PurposeAllows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assaysDepositorInsertmKate2
ExpressionMammalianPromoterSP6Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H sfGFP-R5 AmpR
Plasmid#225139PurposeRecombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 protein fused to silaffin-R5 and sfGFP
TagsHis tagExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Mouse, Human)
UseRetroviralAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad3
Plasmid#89828PurposeKnockdown human Smad3 expression in human mammalian cellsDepositorInsertSMAD3 shRNA (SMAD3 Human)
UseRetroviralAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TTR F87E
Plasmid#238075PurposeBacterial expression of human TTR mutantsDepositorAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC025 (∆CBBp)
Plasmid#226203PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).DepositorInsertHomology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
UseSynthetic BiologyMutation∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpApAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC036 (∆pHG1)
Plasmid#226206PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.DepositorInsertHomology arms for the pHG1 megaplasmid replication region
UseSynthetic BiologyMutation∆helD ∆repA ∆repB ∆parABAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-nCLC
Plasmid#193564PurposeExpresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
TagsmEGFPExpressionMammalianMutationNeuronal isoformPromoterCMVAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
nCLC-ShadowY
Plasmid#193565PurposeExpresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
TagsShadowYExpressionMammalianMutationNeuronal isoformPromoterCMVAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW031
Plasmid#231144PurposeUsed to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.DepositorInsertS. aureus Sortase A mutant
TagsNostoc punctiform split intein C-terminal fragmen…ExpressionBacterialMutationP94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…PromoterT7Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only