We narrowed to 9,982 results for: otos

1. pAAV-Syn-ChromeT-GFP

   Plasmid

   #123316

   Purpose

   AAV-mediated expression of ChromeT-GFP under the Syn promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   Syn

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SOX17-3'gRNA

   Plasmid

   #210465

   Purpose

   gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3/er-(n2)oxStayGold(c4)v2.0

   Plasmid

   #186296

   Purpose

   A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)v2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA Myc DBC1

   Plasmid

   #35096

   Depositor

   Osamu Hiraike

   Article

   Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

   Insert

   deleted in breast cancer 1 (CCAR2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Use

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available Since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. iOn-CAG∞MCS

   Plasmid

   #154013

   Purpose

   Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   MCS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LiOn-CAG∞GFP

   Plasmid

   #154015

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. iOn-CAG∞RFP

   Plasmid

   #154014

   Purpose

   Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LiOn-CAG∞IRFP

    Plasmid

    #154017

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    IRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFIN-RK-GFP-mIRBP156-CHER-WPRE

    Plasmid

    #44351

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Inserts

    RK-GFP
    mIRBP156-CHER
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human rhodopsin kinase (pos 2554-2850) and mouse …

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5HT2C-RNA2-Flag

    Plasmid

    #79679

    Purpose

    serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    serotonin receptor 2C, RNA2 (HTR2C Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

13. 5HT2C-RNA1-FLAG

    Plasmid

    #79678

    Purpose

    serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    5HT2C-RNA1 (HTR2C Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. p55-H2B-Dendra2

    Plasmid

    #80609

    Purpose

    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    H2B-Dendra2 (H2BC21 Human, Synthetic)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVS1-PdTK-CAG-mCh-[uBglII]

    Plasmid

    #107280

    Purpose

    puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

17. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

    Plasmid

    #195049

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPGI

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCGN-HCF-1-N1011Δ382-450

    Plasmid

    #92322

    Purpose

    N-term of HCF-1 protein without amino acids 382-450

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-1011 with internal deletion Δ382-450

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCGN-HCF-1-N450

    Plasmid

    #92321

    Purpose

    N-term 1-450 of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-450. Note that a SRRASGTYGRV amino-terminal …

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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