We narrowed to 9,154 results for: pho

1. pEGFP-FLAG-AKAP-Lbc

   Plasmid

   #67571

   Purpose

   Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   A-kinase anchoring protein Lbc (AKAP13 Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   R2604G in AKAP

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pHR-ZIP(RK)

   Plasmid

   #27136

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Arginines in the SH2 domains mutated to lysines (…

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-CRY2-INPP5E(D556A)

   Plasmid

   #79563

   Purpose

   expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Flag-KLF6-4D (1054)

   Plasmid

   #49490

   Purpose

   expresses human Flag tagged KLF6 with 4D mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   KFL6-4D (KLF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   three serines and one tyrosine mutated to aspart…

   Promoter

   CMV

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pHR-ZIP(FF)

   Plasmid

   #27135

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Tyrosines in ITAMS 1-3 mutated to phenylalanines …

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST26 Ago2 S387E

   Plasmid

   #59906

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   S387E, phospho-dominant

   Promoter

   CMV

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

   Plasmid

   #162880

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1 mTagBFP2 rat PGK1 shRNA

   Plasmid

   #222869

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat PGK1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. flag-hKLF6-4A (1010)

   Plasmid

   #49489

   Purpose

   expresses human Flag tagged KLF6 with 4A mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   KFL6-4A (KLF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   three serines and one tyrosine mutated to alanin…

   Promoter

   CMV

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pDEST26 Ago2 S387A

    Plasmid

    #59905

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    S387A, phospho-null alanine

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

    Plasmid

    #47580

    Purpose

    Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Inserts

    MKK4-EE (MAP2K4 Human)
    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Promoter

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

14. RMCE ABCDE>ala DNA-PKcs

    Plasmid

    #233914

    Purpose

    Low copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs ABCDE phosphorylation site mutant (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    phosphorylation sites 2609, 2612, 2620, 2624, 263…

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCV-IRES_Tomato MEF2C S222D

    Plasmid

    #107231

    Purpose

    hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S222D

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-3_Flag-TET1-S-WT

    Plasmid

    #232942

    Purpose

    Expresses short isoform of TET1 in mammalian cells in its wild-type form

    Depositor

    Jianjun Chen

    Article

    Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

    Insert

    TET1-S-WT (TET1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-SUMO-Rab10 Q68L

    Plasmid

    #236720

    Purpose

    Expresses Rab10 Q68L with His tag and SUMO cleavage sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    Rab10 Q68L (RAB10 Human)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Q68L

    Promoter

    T7

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-3_Flag-TET1-S-MUT

    Plasmid

    #232943

    Purpose

    Expresses mutant form of the short isoform of TET1 in mammalian cells

    Depositor

    Jianjun Chen

    Article

    Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

    Insert

    TET1-S-MUT (TET1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    H1652Y and D1654A

    Promoter

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-no-Flag-TET1-S-WT

    Plasmid

    #232941

    Purpose

    Expresses short isoform of TET1 in mammalian cells in its wild-type form

    Depositor

    Jianjun Chen

    Article

    Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

    Insert

    TET1-S-WT (TET1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge