We narrowed to 9,253 results for: pho

1. ENTP-bio-His

   Plasmid

   #53358

   Purpose

   Expresses full-length Ectonucleoside triphosphate diphosphohydrolase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ENTP (ENTPD1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR2b-PTPN11(D425A,C459S)

   Plasmid

   #215515

   Purpose

   Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   D425A, C459S (Phosphatase inactive)

   Promoter

   none

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. FENEF-Sensor

   Plasmid

   #208930

   Purpose

   in vitro p38-Sensor for luminescent detection of p38-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   FENEF sequence from human ATF2 with phospho site (ATF2 Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

   Plasmid

   #207344

   Purpose

   Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Δ37, Δ115-133, Δ204-217

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSBbi-Pur-Lyn-AktAR2-D

   Plasmid

   #125198

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Article

   Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

   Insert

   Lyn-AktAR2-D
   

   Use

   Transposon

   Tags

   Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DNA-PKcs T3950D

   Plasmid

   #83320

   Purpose

   DNA-PKcs T3950A

   Depositor

   Katheryn Meek

   Article

   Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Activation loop phosphorylation site 3950 substit…

   Promoter

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-FLAG-AKAP-Lbc

   Plasmid

   #67571

   Purpose

   Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   A-kinase anchoring protein Lbc (AKAP13 Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   R2604G in AKAP

   Promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHR-ZIP(RK)

   Plasmid

   #27136

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Arginines in the SH2 domains mutated to lysines (…

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-CRY2-INPP5E(D556A)

   Plasmid

   #79563

   Purpose

   expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Flag-KLF6-4D (1054)

    Plasmid

    #49490

    Purpose

    expresses human Flag tagged KLF6 with 4D mutation

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    KFL6-4D (KLF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    three serines and one tyrosine mutated to aspart…

    Promoter

    CMV

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pHR-ZIP(FF)

    Plasmid

    #27135

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Tyrosines in ITAMS 1-3 mutated to phenylalanines …

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST26 Ago2 S387E

    Plasmid

    #59906

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    S387E, phospho-dominant

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF.DEST51-PTPN11(D425A,C459S)-Clover

    Plasmid

    #215526

    Purpose

    Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    D425A, C459S (Phosphatase inactive)

    Promoter

    EF1a

    Available since

    Sept. 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pENTR2b-PTPN11(D425A,C459S)-Clover

    Plasmid

    #215520

    Purpose

    Gateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry vector

    Tags

    Clover

    Expression

    Mutation

    D425A, C459S (Phosphatase inactive)

    Promoter

    none

    Available since

    Sept. 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

    Plasmid

    #162880

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1 mTagBFP2 rat PGK1 shRNA

    Plasmid

    #222869

    Purpose

    Expresses mTagBFP2 along with an shRNA against rat PGK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. flag-hKLF6-4A (1010)

    Plasmid

    #49489

    Purpose

    expresses human Flag tagged KLF6 with 4A mutation

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    KFL6-4A (KLF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    three serines and one tyrosine mutated to alanin…

    Promoter

    CMV

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pDEST26 Ago2 S387A

    Plasmid

    #59905

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    S387A, phospho-null alanine

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge