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pQCascade-IS2

Plasmid

#140625

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade-tra13

Plasmid

#140628

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-tra13. The crRNA-tra13 targets 13 copies transposon loci in Tatumella citrea.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSZT025

Plasmid

#140033

Purpose

KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Publication

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Publication

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Publication

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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J8AAV-HDR Ctnnb1.1

Plasmid

#73452

Purpose

J8AAV-HDR U6sgCtnnb1.1 template

Depositor

Wen Xue

Publication

Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

AAV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Publication

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-hpGRISZ-TM

Plasmid

#248028

Purpose

Plasmid for packing AAV for hpGRISZ (synaptic zinc sensor)

Depositor

Huiwang Ai

Publication

Zhang et al ACS Sens. 2026 Mar 3. doi: 10.1021/acsse

Insert

hpGRISZ

Use

AAV

Promoter

hSyn

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Publication

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Publication

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pZG61

Plasmid

#202575

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 67kDa

Plasmid

#201372

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(NLS)SV40-EGFP-6PrA

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

Plasmid

#199350

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOstar

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

Plasmid

#199358

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-EGFP

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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P-BT1763

Plasmid

#188111

Purpose

pBolux harboring the promoter region upstream of BT1763

Depositor

Guy Townsend

Publication

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT1763 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT1763

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Publication

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHWBF06exp

Plasmid

#130831

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Publication

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Publication

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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